ISF Sampling in Dermal and Adipose Tissue - dOFM and aOFM

dOFM – dermal OFM

  • Linear push-pull type probes that are membrane free wih macroscopic openings
  • Used for sampling of Peptides, Proteins, Antibodies, Lipophilic Substances, Bound and Unbound Drugs, Transporters, Enzymes, Nanocarriers, Vesicles and Cells
  • Concentrations and action of dermatological drugs (PK/PD) are measured directly in the dermis.
  • Transport and effects of drugs are monitored after topical or systemic application (e.g., topical glucocorticoids and systemic antibodies in psoriatic or inflammatory animal models).

aOFM – adipose OFM

  • Concentrations and action of drugs (PK/PD) are measured directly in adipose tissue.
  • Independent of the mode of administration (oral, subcutaneous, topical, intravenous or other).
  • Can be combined with glucose-clamp techniques to investigate e.g. insulin sensitivity as well as insulin concentrations and the effects in subcutaneous tissue.

 

  • All a/dOFM supplies are gamma-irradiated (sterile).

 

Content coming soon


ISF Sampling in Dermal and Adipose Tissue – dOFM and aOFM

Microperfusion Pump and Accessories

 Item Number  Item Description  Specifications
 OFM-Pump  
 (MPP102PC) 

 Microperfusion Pump Preclinical

 2 x 3 channels

  • Push and pull option integrated in one pump
  • 2 independent controllable pump heads
  • 6 channels (3 per pump head)
  • Up to 3 push and 3 pull channels
  • Standard flow rates: 0.1 - 10 µL/min
  • No flow-rate calibration necessary
  • Attachable to large animals (dog, pig & primate)
  • Perfusion buffer delivered in an enclosed, sealed and gamma irradiated bag
  • Compatible low bind tubing set​  

    Adjustable flow rates: 0.1 - 10μl/min flow rate (with an accuracy of ± 20%)
    Resolution:
    0.1μl/min at 0.1 – 2.0μl/min flow rate
    0.2μl/min at 2.0 – 5.0μl/min flow rate
    0.5μl/min at 5.0 – 10.0μl/min flow rate
    Typical operating time (with a new battery): up to 48 hours at 1.0μl/min and up to 24 hours at 10μl/min.
    Display: LCD with backlight
    Dimensions: 130 x 92 x 34.5mm
    Total weight: 295g excl. battery and accessories.
    Ambient operating temperature: 5 to 40°C

  • Power Supply with main supply or primary battery 
  • Includes power supply
  • Battery not included

 Microperfusion Pump at a glance

 OFM-BAG

 Perfusate Bag- 10 ml

 (pack of 3)

  •  For use with OFM-Pump
  •  Ready for connection to up to 3 push channels
  •  Can be used with single channel OFM-P-110  or 3 channel  OFM-P3-110 push inlet tubings 
  •  Bag can be placed into OFM-Pump or externally 
  •  Perfusate not included

Standard a/d OFM Probe

 Item Number  Item Description  Specifications
 a/d OFM-P-15 

 Linear OFM Probe for   Skin and Adipose Tissue 

 (pack of 3)
 
 

  • Inert & low adsorptive polymer, outer diameter 0.55 mm; inner diameter 0.40 mm, exchange area 15 mm
  • Shaft length: 200 mm
  • Includes Mandrel (stainless steel)
  • Customized diameters available upon request Ask BASi

 Standard a/d OFM Tubing Sets

 Item Number  Item Description  Specifications
 MD-1510

 Flanged Tubing Connectors - clear
 (pack of 20)

  • Soak connectors in 70% ethanol prior to use. Connectors easily slide on, and then shrink as the alcohol evaporates.

 (not gamma-irradiated by default, can be gas sterilized upon request when ordering)


Raturn and Caging Options

 MD-1404  Raturn Microdialysis Stand-Alone System - rat
 MD-1409  Raturn Microdialysis Stand-Alone System - mouse 
 AMD-R Raturn
  Raturn compatible cages 

Automated Refrigerated Fraction Collection 

 MD-1201  Refrigerated Dual Channel Fraction Collector
 MD-1202U  Refrigerated Single Channel Fraction Collector 

 

Content coming soon

 

BASi can assist you with setting up your own OFM platform in your lab. Contact us with your request.
Contact us if you are interested in outsourcing a preclinical OFM study with Joanneum Research.
Click for a/dOFM flyer.
Click for published literature on a/dOFM.
Click for a/dOFM probe user manual.
Click for a/dOFM tubing connections and specs.
Click for OFM pump user manual.
Click for OFM bag user manual.
Read OFM Press Release.

a/dOFM Instructional Videos:

Inserting a/d OFM-P-15

OFM Open Flow Microperfusion Pump Battery Handling

OFM Open Flow Microperfusion Perfusate Bag Handling

OFM Open Flow Microperfusion Tubing Set Insertion Into Pump