ISF Sampling in Dermal and Adipose Tissue - dOFM and aOFM

dOFM – dermal OFM

  • Linear push-pull type probes that are membrane free wih macroscopic openings
  • Used for sampling of Peptides, Proteins, Antibodies, Lipophilic Substances, Bound and Unbound Drugs, Transporters, Enzymes, Nanocarriers, Vesicles and Cells
  • Concentrations and action of dermatological drugs (PK/PD) are measured directly in the dermis.
  • Transport and effects of drugs are monitored after topical or systemic application (e.g., topical glucocorticoids and systemic antibodies in psoriatic or inflammatory animal models).

aOFM – adipose OFM

  • Concentrations and action of drugs (PK/PD) are measured directly in adipose tissue.
  • Independent of the mode of administration (oral, subcutaneous, topical, intravenous or other).
  • Can be combined with glucose-clamp techniques to investigate e.g. insulin sensitivity as well as insulin concentrations and the effects in subcutaneous tissue.

 

  • All a/dOFM supplies are gamma-irradiated (sterile).

 

Content coming soon


ISF Sampling in Dermal and Adipose Tissue – dOFM and aOFM

Microperfusion Pump and Accessories

 Item Number  Item Description  Specifications
 OFM-Pump  
 (MPP102PC) 

 Microperfusion Pump Preclinical

 2 x 3 channels

  • Push and pull option integrated in one pump
  • 2 independent controllable pump heads
  • 6 channels (3 per pump head)
  • Up to 3 push and 3 pull channels
  • Standard flow rates: 0.1 - 10 µL/min
  • No flow-rate calibration necessary
  • Attachable to large animals (dog, pig & primate)
  • Perfusion buffer delivered in an enclosed, sealed and gamma irradiated bag
  • Compatible low bind tubing set​  

    Adjustable flow rates: 0.1 - 10μl/min flow rate (with an accuracy of ± 20%)
    Resolution:
    0.1μl/min at 0.1 – 2.0μl/min flow rate
    0.2μl/min at 2.0 – 5.0μl/min flow rate
    0.5μl/min at 5.0 – 10.0μl/min flow rate
    Typical operating time (with a new battery): up to 48 hours at 1.0μl/min and up to 24 hours at 10μl/min.
    Display: LCD with backlight
    Dimensions: 130 x 92 x 34.5mm
    Total weight: 295g excl. battery and accessories.
    Ambient operating temperature: 5 to 40°C

  • Power Supply with main supply or primary battery 
  • Includes power supply
  • Battery not included

 Microperfusion Pump at a glance

 OFM-BAG

 Perfusate Bag- 10 ml

 (pack of 6)

  •  For use with OFM-Pump
  •  Ready for connection to up to 3 push channels
  •  Can be used with single channel OFM-P-110  or 3 channel  OFM-P3-110 push inlet tubings 
  •  Bag can be placed into OFM-Pump or externally 
  •  Perfusate not included

Standard a/d OFM Probe

 Item Number  Item Description  Specifications
 a/d OFM-P-15 

 Linear OFM Probe for   Skin and Adipose Tissue 

 (pack of 6)
 
 

  • Inert & low adsorptive polymer, outer diameter 0.55 mm; inner diameter 0.40 mm, exchange area 15 mm
  • Shaft length: 200 mm
  • Includes Mandrel (stainless steel)
  • Customized diameters available upon request Ask BASi

 Standard a/d OFM Tubing Sets

 Item Number  Item Description  Specifications
 OFM-PH 

 Single Channel OFM Pump Head Tubing 
 (pack of 6)

  • Single channel tubing with PEEK port for OFM-Pump Head
  • Used for connecting OFM-LT-1000 tubing (pull side)
  • Must be used with connectors MD-1510 

 

 Click here for tubing specs

 OFM-P-110

 Single Channel Push Tubing
 (pack of 6) 

  • 110 mm length
  • For connection from OFM-BAG to pump head (push side)
  • Luer-Lock pre-mounted for use with OFM-BAG / OFM-Pump
  • Pre-mounted with OFM-PH  tubing (push side)
  • Must be used with connectors MD-1510
 
 Click here for tubing specs
 OFM-LT-1000

 Low Bind Tubing
 
1000 mm length 
 (pack of 6)

 For connection from:
 OFM-P-110 or OFM-P3-110 (push tubing) to probe inlet, probe outlet to OFM-PH or OFM-PH3 (pull side) to collection vial 

  • Can be cut to desired length by the user 
  • Must be used with connectors MD-1510

 
 Click here for tubing specs

 OFM-PP1-LT-1000 

 Combined Push Pull Low Bind Tubing 
 
(pack of 6)

  • User for connection to 1 probe
  • Ready to use fully assembled Push-Pull Tubing
  • Can be cut to desired length by the user
 
 Click here for tubing specs
 OFM-PH3

 Three Channel OFM Pump Head Tubing
 (pack of 6)

  • 3 channel tubing with PEEK-Port for OFM-Pump Head 
  • Used for connecting OFM-LT-1000 tubing (pull side)
  • Three channel tubing must be used when all three pump channels are operated at the same time
  • Must be used with connectors MD-1510 

 

 Click here for tubing specs

 OFM-P3-110

 Three Channel Push Tubing
 
(pack of 6)

  • 110 mm length 
  • For connection from OFM-BAG to Pump Head (push side)
  • Luer-Lock pre-mounted for use with OFM-BAG / OFM-Pump
  • Pre-mounted with OFM-PH3 tubing (push side)
  • Three channel tubing can be used when all three pump channels are operated at the same time
  • Must be used with connectors MD-1510

 

 Click here for tubing specs

 MD-1510

 Flanged Tubing Connectors - clear
 (pack of 20)

  • Soak connectors in 70% ethanol prior to use. Connectors easily slide on, and then shrink as the alcohol evaporates.

 (not gamma-irradiated by default, can be gas sterilized upon request when ordering)


Raturn and Caging Options

 MD-1404  Raturn Microdialysis Stand-Alone System - rat
 MD-1409  Raturn Microdialysis Stand-Alone System - mouse 
 AMD-R Raturn
  Raturn compatible cages 

Automated Refrigerated Fraction Collection 

 MD-1201  Refrigerated Dual Channel Fraction Collector
 MD-1202U  Refrigerated Single Channel Fraction Collector 

 

Content coming soon

 

BASi can assist you with setting up your own OFM platform in your lab. Contact us with your request.
Contact us if you are interested in outsourcing a preclinical OFM study with Joanneum Research.
Click for a/dOFM flyer.
Click for published literature on a/dOFM.
Click for a/dOFM probe user manual.
Click for a/dOFM tubing connections and specs.
Click for OFM pump user manual.
Click for OFM bag user manual.
Read OFM Press Release.