cOFM – cerebral OFM 

  • Probes are membrane free push-pull type probes with macroscopic openings
  • Allows sampling of Peptides, Proteins, Antibodies, Lipophilic Substances, Bound and Unbound Drugs, Enzymes, Nanocarriers, Vesicles and Cells
  • Probes allow blood-brain barrier (BBB) re-establishment within 14 days after probe implantation.
  • Allows sampling of drugs and endogenous substances in brain tissue with an intact BBB.
  • Allows continous monitoring of BBB intactness.
  • Allows continuous monitoring of drug transport across the intact BBB.
  • Allows long-term monitoring for up to 30 days.

BASi offers 2 complete cOFM setups:

  1. cOFM in freely moving rodents.
  2. cOFM in anesthetized animals (optimimzed setup for sampling of highly lipophilic or sticky molecules).
  • All cOFM supplies are gamma-irradiated (sterile).

 

Content coming soon

 cOFM-FM – Freely Moving Animal Setup for Sampling in the brain

Microperfusion Pump and Accessories

 Item number  Item Descritption   Specifications and Comments
 OFM-Pump   
 (MPP102PC
)
Microperfusion Pump Preclinical 
 2x3 channels

 - Power Supply with main supply or primary battery 
 - Includes power supply
 - Battery not included

  • Push and pull option integrated in one pump
  • 2 independent controllable pump heads
  • 6 channels (3 per pump head)
  • Up to 3 push and 3 pull channels
  • Standard flow rates: 0.1 - 10 µL/min
  • No flow-rate calibration necessary
  • Attachable to large animals (dog, pig & primate)
  • Perfusion buffer delivered in an enclosed, sealed and gamma irradiated bag
  • Compatible low bind tubing set​  

    Adjustable flow rates: 0.1 - 10μl/min flow rate (with an accuracy of ± 20%)
    Resolution:
    0.1μl/min at 0.1 – 2.0μl/min flow rate
    0.2μl/min at 2.0 – 5.0μl/min flow rate
    0.5μl/min at 5.0 – 10.0μl/min flow rate
    Typical operating time (with a new battery): up to 48 hours at 1.0μl/min and up to 24 hours at 10μl/min.
    Display: LCD with backlight
    Dimensions: 130 x 92 x 34.5mm
    Total weight: 295g excl. battery and accessories.
    Ambient operating temperature: 5 to 40°C

 Microperfusion Pump at a glance

OFM-BAG  Perfusate Bag-10 mL
 (pack of 6)

 - For use with OFM-Pump
 - Ready for connection to up to 3 push channels
 - Can be used with single channel OFM-P-110 or 3 channel OFM-P3-110 push inlet tubings
 - Bag can be placed into OFM-Pump or externally
 - Perfusate not included


Standard cOFM Probe Kits (Guide, Dummy, Sampling Insert, and Locking Wedge)

Item Number Item Description Illustration / Specifications

cOFM-P-6-1

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Concentric OFM Probe Kit for Brain
(6 mm shaft, 1 mm open surface, 7 mm total length)
(pack of 3)

Each pack includes:
1 pc. cOFM guide (X=6 mm, Y=1 mm)
1 pc. cOFM healing dummy cOFM-D-7 (Z=7 mm)
1 pc. cOFM sampling insert  cOFM-S-7 (Z=7 mm)
2 pc. cOFM locking wedge cOFM-Lock

 

 Click here for probe implant illustration 

 cOFM-P-6-2

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Concentric OFM Probe Kit for Brain
(6 mm shaft, 2 mm open surface, 8 mm total length)
(pack of 3)

Each pack includes: 
1 pc. cOFM guide (X=6 mm, Y=2 mm)
1 pc. cOFM healing dummy cOFM-D-8 (Z=8 mm)
1 pc. cOFM sampling insert  cOFM-S-8 (Z=8 mm)
2 pc. cOFM locking wedge cOFM-Lock

 cOFM-P-8-1

Recommended for:
Rat ventral regions

Concentric OFM Probe Kit for Brain
(8 mm shaft, 1 mm open surface, 9 mm total length)
(pack of 3)

Each pack includes: 
1 pc. cOFM guide (X=8 mm, Y=1 mm)
1 pc. cOFM healing dummy cOFM-D-9 (Z=9 mm)
1 pc. cOFM sampling insert  cOFM-S-9 (Z=9 mm)
2 pc. cOFM locking wedge cOFM-Lock

cOFM-P-8-2

Recommended for:
Rat ventral regions

Concentric OFM Probe Kit for Brain
(8 mm shaft, 2 mm open surface, 10 mm total length)
(pack of 3)

Each pack includes:
1 pc. cOFM guide (X=8 mm, Y=2 mm)
1 pc. cOFM healing dummy cOFM-D-10 (Z=10 mm)
1 pc. cOFM sampling insert  cOFM-S-10 (Z=10 mm)
2 pc. cOFM locking wedge cOFM-Lock

Standard cOFM Probe Kits (Guide, Dummy, and Locking Wedge) 

Item Number Item Description Illustration / Specifications

cOFM-GD-6-1

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Concentric OFM Guide / Dummy for Brain
6 mm shaft, 1 mm open surface, Total length 7 mm)
(pack of 3) 
 
Each pack includes: 
1 pc. cOFM guide (X=6 mm, Y=1 mm)
1 pc. cOFM healing dummy cOFM-D-7 (Z=7 mm)
1 pc. cOFM locking wedge cOFM-Lock

Order appropriate Sampling Insert cOFM-S-7  separately

 

 Click here for probe implant illustration 

cOFM-GD-6-2

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Concentric OFM Guide / Dummy for Brain
6 mm shaft, 2 mm open surface, Total length 8 mm)
(pack of 3) 
 
Each pack includes: 
1 pc. cOFM guide (X=6 mm, Y=2 mm)
1 pc. cOFM healing dummy cOFM-D-8 (Z=8 mm)
1 pc. cOFM locking wedge cOFM-Lock

Order appropriate Sampling Insert cOFM-S-8 separately

cOFM-GD-8-1

Recommended for:
Rat ventral regions

Concentric OFM Guide / Dummy for Brain
8 mm shaft, 1 mm open surface, Total length 9 mm)
(pack of 3) 

Each pack includes: 
1 pc. cOFM guide (X=8 mm, Y=1 mm)
1 pc. cOFM healing dummy cOFM-D-9 (Z=9 mm)
1 pc. cOFM locking wedge cOFM-Lock

Order appropriate Sampling Insert cOFM-S-9  separately

cOFM-GD-8-2

Recommended for:
Rat ventral regions

Concentric OFM Guide / Dummy for Brain
8 mm shaft, 2 mm open surface, Total length 10 mm)
(pack of 3) 
 
Each pack includes: 
1 pc. cOFM guide (X=8 mm, Y=2 mm)
1 pc. cOFM healing dummy cOFM-D-10 (Z=10 mm)
1 pc. cOFM locking wedge cOFM-Lock

Order appropriate Sampling Insert cOFM-S-10  separately

 Standard cOFM Dummies

Item Number Item Description Illustration / Specifications

cOFM-D-7

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Healing Dummy   Replacement
(Z=7 mm)
(pack of 6)

 

 Click here for probe implant illustration 

cOFM-D-8 

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Healing Dummy   Replacement
(Z=8 mm)
(pack of 6)

cOFM-D-9

Recommended for:
Rat dorsal regions

Healing Dummy   Replacement
(Z=9 mm)
(pack of 6)

cOFM-D-10

Recommended for:
Rat dorsal regions

Healing Dummy   Replacement
(Z=10 mm)
(pack of 6)

 Standard cOFM Sampling Inserts

Item Number Item Description Illustration / Specifications

cOFM-S-7

Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Sampling Insert   Replacement
(Z=7 mm)
(pack of 6)

 

 Click here for probe implant illustration 

cOFM-S-8


Recommended for:
Mouse dorsal regions
Mouse ventral regions
Rat dorsal regions

Sampling Insert   Replacement
(Z=8 mm)
(pack of 6)

cOFM-S-9

Recommended for:
Rat dorsal regions

Sampling Insert   Replacement
(Z=9 mm)
(pack of 6)

cOFM-S-10

Recommended for:
Rat dorsal regions

Sampling Insert   Replacement
(Z=10 mm)
(pack of 6)

Custom cOFM Probe Kits (Guide, Dummy, Sampling Insert, and Locking Wedge)

Item Number Item Description Illustration / Specifications
 cOFM-P-X-Y

Concentric OFM Probe Kit for Brain 
(Custom Length)

(pack of 3)


Each pack Includes:
1 pc. cOFM guide (X mm, Y=1 or 2 mm)
1 pc. cOFM healing dummy cOFM-D-Z (Z= X+Y mm)
1 pc. cOFM sampling insert cOFM-S-Z (Z=X+Y mm)
2 pc. cOFM locking wedge cOFM-Lock

Ask BASi for customization of X , Y (and Z)

 
 

 Click here for probe implant illustration 

 

 cOFM-GD-X-Y Concentric OFM Guide / Dummy for Brain
(Custom Length) 
(pack of 3) 
 
Each pack includes: 
1 pc. cOFM guide (X mm, Y=1 or 2 mm)
1 pc. cOFM healing dummy cOFM-D-Z (Z=X+Y mm)
1 pc. cOFM locking wedge cOFM-Lock

Order appropriate Sampling Insert cOFM-S-Z separately

Ask BASi for customization of X , Y (and Z)
 cOFM-D-Z

Healing Dummy Replacement
(Custom Length)
(pack of 6)

Ask BASi for customization of Z

 cOFM-S-Z

Sampling Insert Replacement
(Custom Length)
(pack of 6) 

Ask BASi for customization of Z

Other Accessories

Item Number Item Description Illustration / Specifications

cOFM-Lock

 

cOFM Locking Wedge Replacement
(3 packs of 20; 60 total)
 

 

 Click here for probe implant illustration 

cOFM-SPH cOFM Stereotaxic Probe Holder
(Designed to implant cOFM guide/dummy with ease during stereotaxic surgery)
(pack of 6)

 Click here for illustration

 (not gamma-irradiated by default, can be gas sterilized upon request when ordering)

cOFM Tubing Sets

Item Number Item Description Specifications
 OFM-PH  

 Single Channel OFM Pump Head Tubing
 
(pack of 6)  

 








 

- Single channel tubing with PEEK port for OFM-Pump Head
- Used for connecting OFM-LT-1000 tubing (pull side)
- Must be used with connectors MD-1510 

  
 Click here for tubing specs

 OFM-P-110  Single Channel Push Tubing 
 (pack of 6)

- 110 mm length
- For connection from OFM-BAG to pump head (push side)
- Luer-Lock pre-mounted for use with OFM-BAG / OFM-Pump
- Pre-mounted with OFM-PH tubing
- Must be used with connectors MD-1510

 

 Click here for tubing specs

 OFM-LT-1000  Low Bind Tubing 1000 mm Length
(pack of 6)

- For connection from: OFM-P-110 or OFM-P3-110 (push tubing) to probe inlet, probe outlet to OFM-PH or OFM-PH3 (pull side) to collection vial 
- Can be cut to desired length by the user 
- Must be used with connectors MD-1510


 Click here for tubing specs

 OFM-PP1-LT-1000     Combined Push-Pull Low Bind Tubing
 (pack of 6)

- Used for connection to 1 probe
- Ready to use fully assembled Push-Pull tubing
- Can be cut to desired length by user 
 

 Click here for tubing specs

 OFM-PH3  Three Channel OFM Pump Head Tubing
 (pack of 6) 

- 3 channel tubing with PEEK-Port  for OFM-Pump Head 
- Used for connecting OFM-LT-1000 tubing (pull side)
- Three channel tubing must be used when all three pump channels are operated at the same time
- Must be used with connectors MD-1510 
 

 Click here for tubing specs

 OFM-P3-110                        Three Channel Push Tubing 
 (pack of 6)

- 110 mm length 
- For connection from OFM-BAG to Pump Head (push side)
- Luer-Lock pre-mounted for use with OFM-BAG / OFM-Pump
- Pre-mounted with OFM-PH3 tubing
- Three channel tubing must be used when all three pump channels are operated at the same time
- Must be used with connectors MD-1510
 

 Click here for tubing specs

 MD-1510  Flanged Tubing Connectors- clear
 
(pack of 20)

- Soak connectors in 70% ethanol prior to use. Connectors easily slide on, and then shrink as the alcohol evaporates.

 (not gamma-irradiated by default, can be gas sterilized upon request when ordering)


 Raturn and Caging Options

 MD-1404  Raturn Microdialysis Stand-Alone System - rat
 MD-1409  Raturn Microdialysis Stand-Alone System - mouse
 AMD-R Raturn
  Raturn compatible cages 

Automated Refrigerated Fraction Collection 

 MD-1201  Refrigerated Dual Channel Fraction Collector
 MD-1202U  Refrigerated Single Channel Fraction Collector 

cOFM - Anesthetized Animal Setup for ISF Sampling in the Brain

For anesthetized animal setup you will only need the below parts:

  1. Guide, Healing Dummies, Sampling Inserts as above
  2. Tubing set OFM-LT-1000 and connectors MD-1510

 

 

 

Content coming soon

BASi can assist you with setting up your own OFM platform in your lab. Contact us with your request.
Contact us if you are interested in outsourcing a preclinical OFM study with Joanneum Research.
Click for cOFM flyer.
Click for published literature on cOFM.
Click for cOFM probe user manual.
Click for cOFM tubing connections and specs.
Click for OFM pump user manual.
Click to view cOFM video.
Read OFM Press Release.